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Protein Kinase A Detection in Human Urine Samples.
Angela Ragone1, Alessia Salzillo1, Annamaria Spina1
1Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via L. De Crecchio 7, 80138 Naples, Italy.
Journal of Clinical Medicine
|September 28, 2021
Summary
This study explored cAMP-dependent protein kinase A (PKA) in urine. Detectable PKA levels were found in some human urine samples, suggesting potential diagnostic use in laboratory medicine.
Area of Science:
- Biochemistry
- Oncology
- Clinical Diagnostics
Background:
- cAMP-dependent protein kinase A (PKA) is implicated in tumor maintenance and has been proposed as a cancer biomarker.
- Active PKA forms and PKA autoantibodies have been detected in cancer patients' sera, but their diagnostic significance is unclear.
- Existing PKA detection assays lack standardization for laboratory diagnostic procedures.
Purpose of the Study:
- To investigate the presence of PKA in human urine samples.
- To assess the potential of PKA as a diagnostic analyte in urine.
Main Methods:
- Quantitative sandwich ELISA was used to screen 30 human urine samples for PKA.
- Western blotting (WB) was employed to confirm PKA presence in ELISA-positive and negative urine samples.
Main Results:
- Detectable PKA levels were identified in 5 out of 30 screened urine samples using ELISA.
- Two samples showed considerably high PKA concentrations.
- Western blotting confirmed the presence of PKA in certain human urine specimens.
Conclusions:
- This study provides the first evidence of PKA presence in human urine.
- PKA in urine shows potential as a novel diagnostic analyte for laboratory medicine.
- Further research is warranted to establish PKA's clinical utility.
