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Related Experiment Video

Updated: Oct 18, 2025

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
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Current Trends on Protein Driven Bioinks for 3D Printing.

Anabela Veiga1,2, Inês V Silva1, Marta M Duarte1

  • 1CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4169-005 Porto, Portugal.

Pharmaceutics
|September 28, 2021
PubMed
Summary

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Protein-based bioinks are revolutionizing biomedical engineering through 3D extrusion bioprinting. This review details developing these advanced biomaterials, from formulation to application, offering methodological guidelines.

Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Biomaterials Science

Background:

  • Three-dimensional (3D) extrusion bioprinting is a rapidly advancing biomedical technology.
  • Protein-based bioinks, including collagen, gelatin, and decellularized extracellular matrix (dECM), are gaining significant traction.
  • Proteins offer tunable properties, biocompatibility, and biomimicry, crucial for cellular signaling and tissue regeneration.

Purpose of the Study:

  • To provide a comprehensive review of protein-driven bioink development for 3D bioprinting.
  • To outline key stages and parameters in creating novel bioinks.
  • To offer methodological guidelines for researchers in the field.

Main Methods:

  • Review of literature on protein bioink formulations, extraction, and characterization.
Keywords:
bioinkextrusion biorpintingprotein-based

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  • Analysis of crosslinking techniques, co-formulation strategies, and bioprinting parameters.
  • Discussion of cell embedding, in vitro, in vivo, and in situ studies, and applications.

    • Main Results:

    • Detailed examination of popular protein bioink formulations, molecular weights, and extraction methods.
    • Highlighting various crosslinking techniques and their impact on bioink properties.
    • Exploration of cell encapsulation, biological assessments, and potential applications.

    Conclusions:

    • A sequential approach to bioink development, considering pre-processing, processing, and post-processing stages, is essential for optimization.
    • Understanding each parameter's relevance facilitates the creation of advanced, functional bioinks.
    • This review serves as a guide for developing novel protein-based bioinks for diverse biomedical applications.