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Antimicrobial, Antioxidant and Antiproliferative Secondary Metabolites from Inonotus nidus-pici

Zsófia Garádi1,2, Miklós Dékány3, Ágnes M Móricz4

  • 1Department of Pharmacognosy, Semmelweis University, Üllői út. 26, H-1085 Budapest, Hungary.

Molecules (Basel, Switzerland)
|September 28, 2021
PubMed

Insights

This study identifies bioactive compounds from Inonotus nidus-pici, a fungus related to chaga. Several isolated compounds show promising antioxidant, antimicrobial, and cytotoxic activities against cancer cells.

Area of Science:

  • Mycology
  • Natural Product Chemistry
  • Pharmacology

Background:

  • Inonotus nidus-pici is a neglected macrofungus from Central-Eastern Europe, related to the well-known chaga fungus (Inonotus obliquus).
  • The chemical composition and biological activities of I. nidus-pici remain largely unexplored.

Purpose of the Study:

  • To isolate and identify secondary metabolites from the methanol extract of Inonotus nidus-pici.
  • To evaluate the isolated compounds for their antioxidant, antimicrobial, and antiproliferative properties.

Main Methods:

  • Methanol extraction of Inonotus nidus-pici.
  • Isolation and structure elucidation of compounds using NMR and MS spectroscopy.
  • Antioxidant activity assessed via DPPH assay.
  • Antimicrobial activity tested against bacterial and fungal strains.
  • Antiproliferative activity evaluated on human cancer cell lines (MES-SA, MES-SA/Dx5, A431).

Main Results:

  • Five compounds were isolated: citropremide (1), 3,4-dihydroxybenzalacetone (2), lanosterol (3), ergost-6,8,22-trien-3β-ol (4), and ergosterol peroxide (5).
  • 3,4-dihydroxybenzalacetone (2) exhibited notable antioxidant activity.
  • Compounds 2 and 4 displayed moderate antimicrobial effects.
  • Compounds 4 and 5 demonstrated significant cytotoxic activity against tested cancer cell lines.

Conclusions:

  • This study provides the first comprehensive chemical profile of Inonotus nidus-pici.
  • The identified secondary metabolites possess significant antioxidant, antimicrobial, and cytotoxic potential, warranting further investigation for therapeutic applications.
Keywords:
Hymenochaetaceaeantimicrobialantiproliferativeceramidechagadoxorubicin resistantsclerotiumsteroids

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