International Council for Standardization in Haematology (ICSH) recommendations for processing of blood samples for...

Area of Science:

• Clinical Laboratory Science
• Hematology
• Preanalytical Variables

Background:

• Accurate coagulation test results are critical for patient diagnosis and management.
• Standardized preanalytical processing of citrated blood samples is essential for reliable laboratory diagnostics.
• Existing guidelines may not comprehensively cover all aspects of sample processing for coagulation testing.

Purpose of the Study:

• To provide global guidance and recommendations for processing citrated blood samples for coagulation tests.
• To address key preanalytical variables impacting coagulation test accuracy in clinical laboratories.
• To establish standardized procedures for sample transport, storage, and handling.

Main Methods:

• The guidance is based on a review of published data in peer-reviewed literature.
• Expert opinion from the International Council for Standardization in Haematology (ICSH) was incorporated.
• Recommendations cover sample transport, clot detection, centrifugation, storage, interfering substances, and platelet function testing variables.

Main Results:

• Detailed recommendations are provided for sample transport, including pneumatic tube systems.
• Guidance addresses the identification and management of clots in citrated samples.
• Specific recommendations are outlined for centrifugation, primary tube storage, stability, and handling of interfering substances like haemolysis, icterus, and lipaemia.

Conclusions:

• Adherence to these recommendations will improve the quality and reliability of coagulation testing.
• Standardized processing of citrated blood samples is crucial for accurate clinical laboratory diagnostics globally.
• This document serves as a vital resource for clinical laboratories worldwide to optimize preanalytical procedures.