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Related Experiment Video

Updated: Oct 18, 2025

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Published on: June 9, 2022

Comparison of two different decellularization methods for processed nerve allograft.

Han-Vit Kang¹, Jin-Hyung Im², Yang-Guk Chung¹

¹Department of Orthopedic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

Cell and Tissue Banking

|September 28, 2021

Summary

A novel decellularization process for nerve allografts efficiently removes DNA while preserving extracellular matrix. This method is suitable for developing processed nerve allografts, offering a viable alternative to traditional techniques.

Keywords:

Allografts Decellularization Nuclease Peripheral nerve injuries Sodium dodecyl sulfate Triton X-100

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Last Updated: Oct 18, 2025

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

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Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Autologous nerve grafts are the gold standard for peripheral nerve defects.
Processed nerve allografts offer an alternative but face challenges in Korea due to cost and availability of key reagents.
A new decellularization process is needed to overcome limitations of the conventional Hudson method.

Purpose of the Study:

To compare the efficacy of a novel decellularization process using low concentration sodium dodecyl sulfate (SDS) and nuclease against the traditional Hudson method.
To evaluate the preservation of the extracellular matrix (ECM) and removal of cellular DNA.
To assess the functional and histological recovery of nerve allografts in vivo.

Main Methods:

Nerve allografts were decellularized using the Hudson method and a novel SDS/nuclease method.
DNA content was quantified, and ECM structure was analyzed using Masson's trichrome staining and scanning electron microscopy.
A sciatic nerve defect model in rats was used to transplant decellularized allografts, with functional and histological outcomes assessed after 12 weeks.

Main Results:

The novel method significantly removed more DNA compared to the Hudson method.
Both methods successfully preserved the extracellular matrix structure.
No significant differences in functional or histological nerve recovery were observed between the two groups in the in vivo study.

Conclusions:

The novel decellularization process is effective in removing cellular content while preserving ECM structure.
This new method is suitable for the development of processed nerve allografts.
The findings support the potential of the novel method as an alternative to conventional techniques.