Related Experiment Video
Updated: Oct 18, 2025
09:40
Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function
Published on: July 29, 2021
6.7K
Advance in Human Epithelial-Derived Organoids Research
Fengjiao Li1, Peng Zhang1,2, Saizhi Wu1
1The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
Molecular Pharmaceutics
|September 28, 2021
Summary
Organoid culture technology enables the development of complex 3D organoids from human epithelial tissues. These advanced models show promise in drug discovery, disease research, and regenerative medicine.
Area of Science:
- Biotechnology
- Stem Cell Biology
- Tissue Engineering
Background:
- Organoids are complex 3D structures mimicking in vivo organs, derived from various stem cells.
- Adult epithelial stem cell-derived organoids offer enhanced maturity and extensive applications.
- Recent advancements have enabled the establishment of diverse human epithelial organoids.
Purpose of the Study:
- To review current organoid culture systems for human epithelial tissues.
- To discuss the potential applications of organoid technology in biomedical research.
Main Methods:
- Organoid development from adult, embryonic, and pluripotent stem cells.
- Self-organization processes driving organoid formation.
- Culturing techniques for various adult human epithelial tissues.
Main Results:
- Organoids replicate in vivo organ architecture and functionality.
- Established organoid models include intestinal, colon, lung, liver, stomach, breast, and oral mucosal.
- Organoids demonstrate significant research and application potential.
Conclusions:
- Epithelial organoids are valuable tools for drug discovery and personalized medicine.
- Organoid technology facilitates disease mechanism research and regenerative medicine.
- This review highlights the importance of organoid systems in advancing human tissue research.
