Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

13.8K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
13.8K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.6K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.6K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

17.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
17.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

10.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
10.3K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.6K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Redox-Induced Microstructure and Phase Dynamics in Nickel: Insights from In Situ Synchrotron X-ray Diffraction.

Journal of the American Chemical Society·2025
Same author

Pulsed laser deposition assisted epitaxial growth of cesium telluride photocathodes for high brightness electron sources.

Scientific reports·2025
Same author

Lithiation Induced Phases in 1T'-MoTe<sub>2</sub> Nanoflakes.

ACS nano·2024
Same author

Role of ferroelectric polarization during growth of highly strained ferroelectric materials.

Nature communications·2020
Same author

The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy.

Nanoscale·2018
Same author

Chain Breakage in the Supercooled Liquid - Liquid Transition and Re-entry of the λ-transition in Sulfur.

Scientific reports·2018
Same journal

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same journal

Cryo-EM analysis of cooperative conformational changes in the SARS-CoV-2 spike protein trimer.

IUCrJ·2026
Same journal

Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.

IUCrJ·2026
Same journal

How cryoEM has advanced our understanding of bacteriophages and bacteriocins targeting Clostridioides difficile.

IUCrJ·2026
Same journal

CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

IUCrJ·2026
Same journal

Cryo-EM-guided subtractive optimization of a novel VCP/p97 inhibitor.

IUCrJ·2026
See all related articles

Related Experiment Video

Updated: Oct 18, 2025

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

9.2K

Prism-based scanning X-ray microscopy.

Kenneth Evans-Lutterodt1

  • 1National Synchrotron Light Source II, Brookhaven National Laboratory, Building 743, PO Box 5000, Upton, NY 11973-5000, USA.

Iucrj
|September 29, 2021
PubMed
Summary
This summary is machine-generated.

A new prism deflection scanning X-ray microscope offers advanced capabilities. This innovative technology has potential applications in various scientific fields.

Keywords:
X-ray mirrorsX-ray nanoprobesX-ray opticsX-ray prismsscanning X-ray microscopy

More Related Videos

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.2K
High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.5K

Related Experiment Videos

Last Updated: Oct 18, 2025

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

9.2K
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.2K
High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.5K

Area of Science:

  • Microscopy
  • X-ray optics
  • Materials science

Background:

  • Traditional X-ray microscopes face limitations in resolution and scanning speed.
  • Advancements in optics and detection are crucial for next-generation imaging.
  • Prism-based deflection offers a novel approach to X-ray beam manipulation.

Purpose of the Study:

  • To introduce and provide context for a novel prism deflection scanning X-ray microscope.
  • To discuss the technical principles underlying this new microscopy technique.
  • To explore potential future applications and research directions.

Main Methods:

  • Commentary on the design principles of prism deflection scanning X-ray microscopy.
  • Discussion of the optical configuration and scanning mechanism.
  • Analysis of the advantages over existing X-ray microscopy methods.

Main Results:

  • The commentary highlights the unique beam-steering capabilities of prism deflection.
  • It emphasizes the potential for high-resolution, rapid scanning X-ray imaging.
  • The discussion provides a theoretical framework for understanding its performance.

Conclusions:

  • The prism deflection scanning X-ray microscope represents a significant technological advancement.
  • Its unique properties suggest broad applicability in fields requiring high-resolution elemental and chemical mapping.
  • Further research and development are expected to unlock its full potential in diverse scientific investigations.