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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
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Application-based guidelines for best practices in plant flow cytometry.

Elwira Sliwinska1, João Loureiro2, Ilia J Leitch3

  • 1Laboratory of Molecular Biology and Cytometry, Department of Agricultural Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|September 29, 2021
PubMed
Summary
This summary is machine-generated.

Flow cytometry (FCM) provides essential data on plant nuclear DNA content, ploidy, and genome size. This guide offers best practices for accurate results in diverse plant science applications.

Keywords:
DNA base compositionDNA contentcell cycleendoreduplicationflow cytometric seed screeninggenome sizein vitro culturesintraspecific variationploidy

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Area of Science:

  • Plant Biology
  • Cytometry

Background:

  • Flow cytometry (FCM) is the primary method for determining nuclear DNA content in plants.
  • Increased accessibility of flow cytometers has led to a rise in new users in plant science.

Purpose of the Study:

  • To provide comprehensive best practice recommendations for FCM in plant science.
  • To address the growing need for standardized FCM protocols and data interpretation.

Main Methods:

  • Detailed discussion of FCM applications for plant ploidy, genome size, DNA base composition, cell cycle activity, and endoreduplication.
  • Inclusion of troubleshooting advice for sample preparation, measurement, and data handling.

Main Results:

  • Advantages and limitations of FCM for various plant analyses are outlined.
  • Specific recommendations for obtaining accurate and reliable FCM data are provided.

Conclusions:

  • This paper serves as a crucial resource for optimizing FCM use in plant systematics, ecology, breeding, and other fields.
  • Best practice recommendations and guidance on reporting FCM data are presented to enhance research quality.