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Related Experiment Video

Updated: Oct 18, 2025

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Versatile and rapid microfluidics-assisted antibody discovery.

Ramona Gaa1, Emmanuel Menang-Ndi2, Shruti Pratapa3

  • 1Protein Engineering and Antibody Technologies, Merck Healthcare KGaA, Darmstadt, Germany.

Mabs
|September 29, 2021
PubMed
Summary

Microfluidic technology accelerates antibody discovery by enabling rapid screening of antibody-secreting cells. This method significantly shortens the time to identify therapeutic antibodies for various diseases.

Keywords:
ASCsCyto-MineMicrofluidicsantibodiesfunctional screening

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Area of Science:

  • Biotechnology
  • Immunology
  • Drug Discovery

Background:

  • Microfluidic applications have advanced antibody discovery in academic and pharmaceutical research.
  • Microfluidics facilitates native chain-paired library generation and direct screening of antibody-secreting cells.
  • Existing methods have identified antibodies for infectious diseases, but microfluidics can expedite discovery for cancer and immunological diseases.

Purpose of the Study:

  • To evaluate a commercial microfluidic device (Cyto-Mine) for rapid antibody identification.
  • To demonstrate the device's capability for screening natively paired antibodies from various sources.
  • To showcase the first reported use of microfluidics for broad functional IgG antibody screening in droplets.

Main Methods:

  • Utilized the Cyto-Mine microfluidic device for antibody discovery.
  • Screened for antibody binding to recombinant antigens and cells expressing target proteins.
  • Performed direct broad functional IgG antibody screening in droplets.

Main Results:

  • Achieved rapid identification of natively paired antibodies from rodent and human samples.
  • Successfully screened antibodies against specific antigens and cellular targets.
  • Demonstrated functional IgG antibody screening in droplets, a novel application.
  • Reduced the process time from cell preparation to confirmed recombinant antibodies to four weeks.

Conclusions:

  • Microfluidic devices like Cyto-Mine significantly accelerate antibody hit discovery.
  • This technology enhances the identification of therapeutic antibodies for diverse indications.
  • The methodology offers a rapid and efficient approach for pharmaceutical antibody development.