Simple electrochemical detection of Listeria monocytogenes based on a surface-imprinted polymer-modified electrode
Qingcao Li1, Zhen Guo2, Xuedan Qiu1
1Clinical Laboratory of Ningbo Medical Centre Lihuili Hospital, Ningbo University, 1111 Jiangnan Street, Ningbo, Zhejiang 315040, PR China. lhlyywqp@163.com.
Abstract:
Listeria monocytogenes (LM) is a foodborne pathogen, and it can pose a risk of serious diseases to the human health. Hence, the development of an effective method for the detection of LM is very important. In this study, by selecting LM as the template and 3-thiopheneacetic acid as the functional monomer, an LM-imprinted polymer (LIP)-based sensor was proposed for the first time to detect LM by electropolymerizing TPA on the glassy carbon electrode (GCE) surface in the presence of LM. After the removal of the LM template from the electrode surface, the obtained sensor was denoted as LIP/GCE, which could effectively recognize and capture LM cells. By using [Fe(CN)6]4-/3- as the probe, its peak current at LIP/GCE could be restricted when the LM cells were captured into the imprinted cavity of LIP/GCE, and the current value decreased with an increase in the LM concentration. Serious conditions were optimized for achieving highly sensitive detection, and a low detection limit (6 CFU mL-1) coupled with a wide linear range (10 to 106 CFU mL-1) was obtained for LM. Finally, the inter-electrode reproducibility, stability, selectivity, and applicability of LIP/GCE were also investigated, and the obtained results were acceptable.
Insights
A novel sensor was developed for detecting Listeria monocytogenes (LM), a dangerous foodborne pathogen. This sensor uses a molecularly imprinted polymer to specifically capture and detect LM with high sensitivity and a low detection limit.
Area of Science:
- Electrochemistry
- Biosensing
- Food Safety
Background:
- Listeria monocytogenes (LM) is a significant foodborne pathogen posing serious health risks.
- Effective detection methods for LM are crucial for public health and food safety.
Purpose of the Study:
- To develop a novel, highly sensitive sensor for the detection of Listeria monocytogenes.
- To utilize molecular imprinting technology for specific LM recognition.
Main Methods:
- Electropolymerization of 3-thiopheneacetic acid on a glassy carbon electrode (GCE) in the presence of LM as a template.
- Fabrication of an LM-imprinted polymer (LIP) sensor (LIP/GCE) for selective LM capture.
- Electrochemical detection of LM using [Fe(CN)6]4-/3- as a redox probe, monitoring changes in peak current.
Main Results:
- The developed LIP/GCE sensor demonstrated effective recognition and capture of LM cells.
- A decrease in the [Fe(CN)6]4-/3- peak current correlated with increased LM concentration.
- Achieved a low detection limit of 6 CFU mL-1 and a wide linear range of 10 to 10^6 CFU mL-1 for LM detection.
- The sensor exhibited acceptable inter-electrode reproducibility, stability, and selectivity.
Conclusions:
- The proposed LM-imprinted polymer sensor offers a promising approach for sensitive and selective detection of Listeria monocytogenes.
- This electrochemical sensor provides a viable tool for monitoring LM in food safety applications.
