Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

45.5K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
45.5K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

279
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
279
Bacterial Phylum Tenericutes01:24

Bacterial Phylum Tenericutes

174
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
174
Prokaryotic Gene Structure and Organization01:28

Prokaryotic Gene Structure and Organization

1000
Prokaryotic genomes exhibit a streamlined organization of coding and non-coding regions essential for gene expression and protein synthesis. While coding regions contain the genetic instructions for proteins or functional RNAs, non-coding regions regulate the precise transcription and translation of these genes.Coding Regions: Proteins and RNAsThe primary coding regions, known as structural genes, include sequences transcribed into messenger RNA (mRNA) and ultimately translated into...
1000
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

8.5K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
8.5K
Bacterial Phylum Proteobacteria01:26

Bacterial Phylum Proteobacteria

260
Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
260

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complete genome sequence of <i>Serratia plymuthica</i> EIF4, a prodigiosin-producing isolate from freshwater.

Microbiology resource announcements·2026
Same author

Comprehensive multi-analytical characterization of a novel Arthrobacter strain producing natural bacterioruberin for food applications.

Food microbiology·2026
Same author

Pyocyanin produced by <i>Pseudomonas aeruginosa</i> creates legacy effects that promote antibiotic resistance evolution in enterococci.

Antimicrobial agents and chemotherapy·2026
Same author

Identification and characterization of pumicyclin A, a novel circular bacteriocin from <i>Bacillus pumilus</i> with a dispensable leader peptide.

mBio·2026
Same author

Dietary allyl-isothiocyanate affects male triglyceride levels in <i>Drosophila melanogaster</i> without detectable changes in microbiota composition.

Frontiers in microbiology·2026
Same author

Complete genome sequence of <i>Janthinobacterium lividum</i> EIF5 isolated from soil of a temperate broadleaf and mixed forest.

Microbiology resource announcements·2026

Related Experiment Video

Updated: Oct 18, 2025

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
06:45

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Published on: January 18, 2014

8.7K

The Bacillus subtilis Minimal Genome Compendium.

Stephan Michalik1, Alexander Reder1, Björn Richts2

  • 1C_FunGene, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, 17487 Greifswald, Germany.

ACS Synthetic Biology
|September 29, 2021
PubMed
Summary

Researchers created a database of 105 annotated genomes from minimal genome strains of Bacillus subtilis. This resource aids in understanding cellular components and their interactions for basic science and biotechnology applications.

Keywords:
Bacillus subtilisMiniBacillusessential genesgenome reductionstrain compendium

More Related Videos

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.6K
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

20.7K

Related Experiment Videos

Last Updated: Oct 18, 2025

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
06:45

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Published on: January 18, 2014

8.7K
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.6K
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

20.7K

Area of Science:

  • Microbiology
  • Genomics
  • Synthetic Biology

Background:

  • Understanding cellular life requires deciphering component contributions and interactions.
  • Minimal genomes are crucial tools for studying these cellular mechanisms.
  • Bacillus subtilis is an industrially relevant model bacterium.

Purpose of the Study:

  • To create a comprehensive database of annotated genomes for a series of Bacillus subtilis strains with sequential genome deletions.
  • To support the MiniBacillus project by providing a valuable genomic resource.
  • To facilitate research in basic science and biotechnology.

Main Methods:

  • Generation of 105 fully annotated genomes from sequential deletion strains of Bacillus subtilis.
  • Sequencing of key intermediate strains to support annotation.
  • Integration of literature knowledge for annotating deletion scars and their effects.

Main Results:

  • A database of 105 annotated genomes representing the MiniBacillus project strain compendium.
  • Characterization of Bacillus subtilis strains with up to 40% genome reduction (e.g., B. subtilis PG38).
  • Detailed annotation including deletion scars and their potential functional impact.

Conclusions:

  • The presented genome library is a comprehensive resource for the MiniBacillus project.
  • This resource will enhance the application of minimal genome strains in scientific research.
  • Facilitates deeper understanding of cellular functions and potential biotechnological advancements.