Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tumor Progression02:07

Tumor Progression

6.6K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.6K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

13.3K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
13.3K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

5.1K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
5.1K
Abnormal Proliferation02:23

Abnormal Proliferation

4.7K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Lineage Tracing And Single-cell Analysis Reveal Proliferative Prom1+ Tumour-propagating Cells And Their Dynamic Cellular Transition During Liver Cancer Progression.

Lineage tracing and single-cell analysis reveal proliferative Prom1+ tumour-propagating cells and their dynamic cellular transition during liver cancer progression.

Lei Zhou1,2, Ken Ho Yu1,3, Tin Lok Wong1,4

  • 1School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.

Gut
|September 30, 2021

Related Experiment Videos

Isolation of CD133+ Liver Stem Cells for Clonal Expansion
12:06

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Published on: October 10, 2011

22.6K
In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

617
Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
10:35

Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice

Published on: September 25, 2013

36.5K

View abstract on PubMed

Summary
This summary is machine-generated.

Prominin-1 (Prom1) marks liver cancer stem cells (CSCs) in hepatocellular carcinoma (HCC). These Prom1+ cells drive tumor growth and heterogeneity, and their depletion hinders cancer progression, offering new therapeutic targets.

Area of Science:

  • Oncology
  • Stem Cell Biology
  • Genomics

Background:

  • Hepatocellular carcinoma (HCC) exhibits significant intratumoral heterogeneity, leading to therapeutic resistance and recurrence.
  • Prominin-1 (PROM1)/CD133 is identified as a key marker for liver cancer stem cells (CSCs) in human HCC.

Purpose of the Study:

  • To investigate the heterogeneity and properties of Prominin-1 positive (Prom1+) cells within HCC using intact mouse models.
  • To elucidate the role of Prom1+ cells in HCC progression and therapeutic resistance.

Main Methods:

  • Establishment of two distinct mouse models for chronic fibrotic and rapid steatosis-related HCC.
  • In vivo lineage tracing and targeted cell depletion using genetically modified mouse lines (Prom1C-L/+; Rosa26tdTomato/+ and Prom1C-L/+; Rosa26DTA/+).
Keywords:
hepatocellular carcinomastem cells

Related Experiment Videos

Isolation of CD133+ Liver Stem Cells for Clonal Expansion
12:06

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Published on: October 10, 2011

22.6K
In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

617
Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
10:35

Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice

Published on: September 25, 2013

36.5K
  • Single-cell RNA sequencing (scRNA-seq) to analyze the transcriptomic profile of Prom1+ cells.

    • Main Results:

    • Prom1+ cells in HCC tumors function as proliferative, tumor-propagating cells with CSC-like properties and undergo clonal expansion in situ.
    • These cells demonstrate increased tumorigenicity in 3D culture and allotransplantation, with the potential to form diverse cancer lineages.
    • Depletion of Prom1+ cells significantly impedes tumor growth and reduces malignant characteristics in both HCC models.
    • scRNA-seq revealed heterogeneity within Prom1+ HCC cells, showing a trajectory towards dedifferentiation with high proliferation and stem cell traits.
    • A conserved gene signature in Prom1 lineage cells predicts poor prognosis in human HCC.
    • Activated oxidant detoxification pathways were identified as a mechanism supporting dedifferentiation and lineage propagation.

    Conclusions:

    • The study reveals the heterogeneity and dynamic behavior of Prom1+ HCC cells using in vivo lineage tracing and scRNA-seq.
    • Prom1+ cells are crucial drivers of HCC progression, exhibiting CSC-like properties and contributing to tumor heterogeneity.
    • Targeting Prom1+ cells presents a promising therapeutic strategy for overcoming resistance and recurrence in HCC.