Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Staphylococcus aureus-derived chemoattractant activity for human monocytes.

A Rot, L E Henderson, E J Leonard

    Journal of Leukocyte Biology
    |July 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Finding Order in Chaos: Quantitative Predictors of Chaos Terrain Morphology on Europa.

    Geophysical research letters·2022
    Same author

    Enhanced Phosphorylation of Bax and Its Translocation into Mitochondria in the Brains of Individuals Affiliated with Alzheimer's Disease.

    The open neurology journal·2018
    Same author

    Efficacy of the entomopathogenic fungus Metarhizium brunneum in controlling the tick Rhipicephalus annulatus under field conditions.

    Veterinary parasitology·2014
    Same author

    The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer.

    British journal of cancer·2014
    Same author

    On-host control of the brown dog tick Rhipicephalus sanguineus Latreille (Acari: Ixodidae) by Metarhizium brunneum (Hypocreales: Clavicipitaceae).

    Veterinary parasitology·2012
    Same author

    The biochemistry and biology of the atypical chemokine receptors.

    Immunology letters·2012
    Same journal

    Correction to: IL-27 signaling negatively regulates FcɛRI-mediated mast cell activation and allergic response.

    Journal of leukocyte biology·2026
    Same journal

    Autofluorescence enables detection and quantification of EETosis in human leukocyte cultures.

    Journal of leukocyte biology·2026
    Same journal

    Cold Atmospheric Plasma as an Immunomodulator: Suppression of T-cell Hyperactivation and Graft-versus-Host Disease via Redox Regulation.

    Journal of leukocyte biology·2026
    Same journal

    Distinct lymphocyte immune signatures to nivolumab and recombinant IL-7 ex vivo in patients with sepsis.

    Journal of leukocyte biology·2026
    Same journal

    Eosinophil Essentials: Identification and Assessment of Activity.

    Journal of leukocyte biology·2026
    Same journal

    Immune cell landscape reveals 5 immune-related subtypes and molecular characteristics with prognostic and therapeutic implications in pan-cancer.

    Journal of leukocyte biology·2026
    See all related articles

    Bacterial products attract immune cells. Staphylococcus aureus culture fluid contains peptides, distinct from fMet-Leu-Phe, that are potent chemoattractants for human monocytes, recruiting them more effectively than the prototype attractant.

    Area of Science:

    • Immunology
    • Microbiology
    • Biochemistry

    Background:

    • Bacterial products are recognized as potent chemoattractants for mammalian leukocytes.
    • Small peptides are suggested as the primary bacterial attractants.
    • N-formylated peptides, like fMet-Leu-Phe, are considered prototype bacterial chemoattractants.

    Purpose of the Study:

    • To compare the chemoattractant properties of Staphylococcus aureus culture fluids with the prototype bacterial attractant fMet-Leu-Phe.
    • To characterize the peptides responsible for the chemotactic activity in S. aureus filtrates.
    • To determine the relative contribution of different peptides to monocyte recruitment.

    Main Methods:

    • Chemotactic activity of S. aureus culture filtrates for human monocytes was assessed using multiwell chemotaxis chambers.

    Related Experiment Videos

  • Gel filtration and susceptibility to proteolytic digestion were used to determine the molecular size and nature of the active peptides.
  • Reverse-phase high-pressure liquid chromatography (HPLC) was employed to separate and quantify individual chemotactic peptide peaks.

    • Main Results:

    • S. aureus culture filtrate exhibited significantly higher chemotactic activity for human monocytes compared to fMet-Leu-Phe.
    • The chemotactic activity was attributed to peptides in the molecular size range of 500-2,000 daltons.
    • HPLC analysis revealed multiple active peptide peaks, with one accounting for 40% of the total activity; these individual peaks also showed superior monocyte recruitment compared to fMet-Leu-Phe.
    • Only a small fraction (5%) of the total S. aureus chemotactic activity co-eluted with fMet-Leu-Phe.

    Conclusions:

    • The potent chemotactic activity of Staphylococcus aureus culture fluid for human monocytes is mediated by peptides distinct from fMet-Leu-Phe.
    • These S. aureus-derived peptides are more effective at recruiting monocytes than the prototype chemoattractant fMet-Leu-Phe.
    • The findings highlight the diversity of bacterial chemoattractants and their differential efficacy in modulating immune cell responses.