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Hepatocellular carcinoma progression mediated by hepatitis B virus-encoded circRNA HBV_circ_1 through interaction

Min Zhu1, Zi Liang1, Jun Pan1

  • 1School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.

Molecular Therapy. Nucleic Acids
|September 30, 2021
PubMed

Insights

Hepatitis B virus (HBV) produces a novel circular RNA (circRNA), HBV_circ_1, which promotes hepatocellular carcinoma (HCC) progression by interacting with CDK1. This finding offers a new therapeutic target for HBV-related liver cancer.

Area of Science:

  • Hepatology
  • Molecular Biology
  • Oncology

Background:

  • Hepatitis B virus (HBV) produces circular RNAs (circRNAs) with largely unknown functions.
  • Hepatocellular carcinoma (HCC) is a major global health concern, often linked to HBV infection.

Purpose of the Study:

  • To identify and characterize novel HBV-derived circRNAs in HCC.
  • To investigate the role of HBV_circ_1 in HCC development and progression.
  • To explore the molecular mechanism of HBV_circ_1 in HCC.

Main Methods:

  • Identification of HBV_circ_1 in HBV-positive cells and HCC tissues.
  • Microarray analysis of HCC samples.
  • In vitro cell proliferation, migration, invasion, and apoptosis assays.
  • In vivo tumor growth studies.
  • Interaction studies between HBV_circ_1 and CDK1.

Main Results:

  • A novel circRNA, HBV_circ_1, was identified and found to be upregulated in HCC tissues.
  • Higher HBV_circ_1 levels correlated with lower patient survival rates.
  • HBV_circ_1 enhanced HCC cell proliferation, migration, invasion, and inhibited apoptosis in vitro.
  • HBV_circ_1 expression increased tumor size in vivo.
  • HBV_circ_1 interacts with CDK1 to promote cell proliferation.

Conclusions:

  • HBV_circ_1 is a novel oncogenic circRNA produced by HBV that promotes HCC progression.
  • The interaction between HBV_circ_1 and CDK1 is a key mechanism driving HCC.
  • HBV_circ_1 represents a potential therapeutic target for HBV-related HCC.

Keywords:
HBV_circ_1circular RNAcyclin-dependent kinasehepatitis B virushepatocellular carcinoma

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