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Updated: Oct 18, 2025

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
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A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

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KRT81 Knockdown Inhibits Malignant Progression of Melanoma Through Regulating Interleukin-8

Kun Zhang1, Yan Liang1, Wancong Zhang1

  • 1Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Plastic Surgery Institute of Shantou University Medical College, Shantou, Guangdong, P.R. China.

DNA and Cell Biology
|September 30, 2021
PubMed

Insights

Keratin 81 (KRT81) is overexpressed in melanoma, promoting cancer progression. Downregulating KRT81 inhibits melanoma cell growth, invasion, and enhances chemotherapy sensitivity, suggesting KRT81 as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Dermatology

Background:

  • Keratin 81 (KRT81) is implicated in various human cancers.
  • The role of KRT81 in melanoma development and progression remains largely unexplored.

Purpose of the Study:

  • To investigate the expression and function of KRT81 in melanoma.
  • To determine the potential of KRT81 as a therapeutic target for melanoma.

Main Methods:

  • Analysis of KRT81 expression in melanoma tissues and cell lines.
  • Functional studies involving KRT81 knockdown in A375 melanoma cells (in vitro and in vivo).
  • Assessment of cell proliferation, colony formation, migration, invasion, apoptosis, and chemosensitivity.
  • Exploration of the underlying molecular mechanisms, focusing on inflammatory cytokine interleukin-8 (IL-8).

Main Results:

  • KRT81 expression was significantly upregulated in melanoma tissues and cell lines compared to non-tumor controls.
  • KRT81 knockdown inhibited melanoma cell proliferation, colony formation, migration, and invasion, while promoting apoptosis.
  • In vivo studies demonstrated that KRT81 knockdown suppressed xenograft tumor growth.
  • KRT81 knockdown enhanced the sensitivity of A375 cells to cisplatin (DDP) and led to the downregulation of IL-8.

Conclusions:

  • KRT81 plays a crucial role in melanoma progression.
  • Downregulation of KRT81 inhibits melanoma growth and metastasis, potentially by modulating IL-8.
  • KRT81 emerges as a promising therapeutic target for melanoma treatment.
Keywords:
IL-8KRT81invasionmelanomamigrationproliferation

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