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YY1 and RTCB in mouse uterine decidualization and embryo implantation
Ran Li1, Xiao-Tong Song1, Si-Wei Guo1
1The Key Laboratory of Animal Resistant Biology of Shandong Province, College of Life Science, Shandong Normal University, Jinan, China.
Reproduction (Cambridge, England)
|September 30, 2021
Summary
The transcription factor YY1 binds in vivo to the Rtcb gene promoter during early pregnancy. This study suggests both YY1 and RNA ligase RTCB are involved in mouse uterine decidualization and embryo implantation.
Area of Science:
- Reproductive biology
- Molecular genetics
- Developmental biology
Background:
- Yin Yang 1 (YY1) is a transcription factor crucial for embryonic development.
- RNA ligase, cyclophilin B (RTCB), is involved in tRNA maturation and Xbp1 mRNA splicing.
- YY1 can regulate Rtcb promoter activity in vitro, but in vivo regulation and roles in early pregnancy are unknown.
Purpose of the Study:
- To investigate the in vivo binding of YY1 to the Rtcb promoter in the mouse uterus during early pregnancy.
- To explore the potential roles of YY1 and RTCB in uterine decidualization and embryo implantation.
Main Methods:
- In vivo chromatin immunoprecipitation (ChIP) assays in mouse uterine tissue.
- Construction and analysis of various animal models to study gene function.
- Assessment of decidualization and embryo implantation processes.
Main Results:
- YY1 was confirmed to bind to the proximal promoter of Rtcb in the mouse uterus during early pregnancy.
- Animal models indicated that both YY1 and RTCB play roles in uterine decidualization.
- Evidence suggests involvement of YY1 and RTCB in embryo implantation during early pregnancy.
Conclusions:
- YY1 directly regulates Rtcb expression in vivo in the pregnant mouse uterus.
- YY1 and RTCB are potentially important factors in the maternal-fetal dialogue, influencing key events of early pregnancy like decidualization and implantation.
