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Related Experiment Video

Updated: Oct 18, 2025

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

DNA template strand segregation in developing zebrafish.

Stella M K Glasauer¹, Therese Triemer², Anne B Neef²

¹Department of Chemistry, University of Zurich, 8057 Zurich, Switzerland; Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara CA 93106, USA.

Cell Chemical Biology

|September 30, 2021

Summary

This summary is machine-generated.

Asymmetric DNA inheritance was studied in zebrafish development. Researchers found no evidence supporting the immortal strand hypothesis, suggesting it doesn't prevent mutation accumulation in stem cells.

Keywords:

DNA metabolic labeling azide-alkyne "click" cycloaddition cell division developmental biology eye development fluorescence microscopy stem cells

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Last Updated: Oct 18, 2025

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Area of Science:

Developmental Biology
Cell Biology
Genetics

Background:

The immortal strand hypothesis proposes asymmetric DNA segregation to minimize mutations in stem cells.
This hypothesis links sister chromatid inheritance to daughter cell fates.

Purpose of the Study:

To investigate DNA template strand segregation during zebrafish development.
To test the immortal strand hypothesis in vivo using live imaging.

Main Methods:

Utilized (2'S)-2'-deoxy-2'-fluoro-5-ethynyluridine (F-ara-EdU) and bromodeoxyuridine (BrdU) for DNA labeling.
Employed light sheet microscopy for high-resolution, whole-embryo imaging of DNA segregation in zebrafish.
Tracked embryonic DNA dynamics throughout larval development in key stem cell niches.

Main Results:

Observed rapid depletion of older DNA template strands from stem cell niches (retina, brain, intestine) during larval development.
Identified prolonged label retention in quiescent progenitors that re-enter replication later.
Found no evidence of asymmetric template strand segregation in over 100 observed daughter cell pairs.

Conclusions:

Asymmetric DNA segregation, as predicted by the immortal strand hypothesis, does not appear to occur in developing zebrafish.
The hypothesis that asymmetric segregation minimizes mutational burden in stem cells is unlikely to apply to zebrafish development based on these findings.