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Updated: Oct 18, 2025

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
Published on: October 18, 2019
Evaluation of polymerase chain reaction-based open reading frame typing method for the clonality investigation of
Yusuke Ota1, Natsune Hongou1, Yoko Nukui2
1Department of Molecular Microbiology, Graduate School of Medicine and Dental Science, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Abstract:
Here, we assessed the utility of a polymerase chain reaction-based open reading frame typing assay for investigating the clonality of Clostridioides difficile isolates. This assay has a higher discriminatory power than multi-locus sequence typing for molecular epidemiological analysis of C. difficile isolates and can provide additional information about toxin genotypes.
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