Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immuno-oncology in the perioperative setting: the next frontier in resectable head and neck cancers - a systematic review.

BMC cancer·2026
Same author

Integrating Metronomic Therapy With Standard Chemotherapy in Advanced Unresectable Head and Neck Cancer: A Randomized Trial Addressing Global Cancer Care Equity (METRO PLUS).

JCO global oncology·2026
Same author

10-Year Outcomes of SAPIEN 3 Transcatheter Aortic Valve Replacement or Surgery in Intermediate-Risk Patients.

Journal of the American College of Cardiology·2026
Same author

Learning dynamical systems with biochemically informed neural ordinary differential equations.

bioRxiv : the preprint server for biology·2026
Same author

Prognostic Significance of Her-2/Neu and Ki-67 Expression in Gallbladder Carcinoma: A Clinicopathological Study Across Resectable and Advanced Stages.

Cureus·2026
Same author

Clinical Applications of Artificial Intelligence in Structural Heart Disease.

Journal of the Society for Cardiovascular Angiography & Interventions·2026

Related Experiment Video

Updated: Oct 18, 2025

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

10.8K

Dramatic transcriptomic differences in Macaca mulatta and Macaca fascicularis with Plasmodium knowlesi infections.

Anuj Gupta1, Mark P Styczynski2, Mary R Galinski3,4

  • 1The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.

Scientific Reports
|October 1, 2021
PubMed
Summary

This study reveals distinct host responses to Plasmodium knowlesi malaria in two macaque species. Differences in immune signaling pathways and inflammation control influence infection outcomes, offering insights for new malaria drug targets.

More Related Videos

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
10:22

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Published on: December 4, 2015

9.0K
Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization FISH
13:06

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization FISH

Published on: October 7, 2012

15.7K

Related Experiment Videos

Last Updated: Oct 18, 2025

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

10.8K
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
10:22

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Published on: December 4, 2015

9.0K
Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization FISH
13:06

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization FISH

Published on: October 7, 2012

15.7K

Area of Science:

  • Malaria research
  • Parasitology
  • Immunology

Background:

  • Plasmodium knowlesi causes significant zoonotic malaria in Southeast Asia.
  • Understanding host-parasite interactions is crucial for controlling severe malaria and fatalities.
  • Limited knowledge exists on molecular mechanisms of P. knowlesi infections, potentially applicable across Plasmodium species.

Purpose of the Study:

  • To compare P. knowlesi sporozoite-initiated infections in Macaca mulatta and Macaca fascicularis.
  • To identify transcriptomic differences in host responses during infection.
  • To elucidate molecular mechanisms underlying differential disease outcomes.

Main Methods:

  • Whole blood RNA-sequencing and transcriptomic analysis were employed.
  • Comparative analysis of P. knowlesi infections in two macaque models.
  • Investigation of host gene expression profiles during liver and blood stages of infection.

Main Results:

  • Transcriptomic differences were observed as early as the liver phase, with distinct signaling pathway activation in M. fascicularis compared to M. mulatta.
  • Initial immune responses during the acute blood stage were similar, but diverged significantly later.
  • M. mulatta exhibited prolonged inflammation and IL10 effects, while M. fascicularis showed increased cell proliferation, correlating with recovery.

Conclusions:

  • Differential host transcriptomics, including inflammation control and cell proliferation pathways, dictate P. knowlesi infection outcomes in macaques.
  • Timely detection and management of inflammation are key for containing P. knowlesi in M. fascicularis.
  • Identified genes and pathways represent potential targets for novel anti-malarial drugs to aid recovery from acute malaria.