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Determination of bovine enamel protein by enzyme-linked immuno-adsorbent assay
Archives of Oral Biology
|January 1, 1986
Abstract:
A specific enzyme-linked immuno-adsorbent assay for enamel protein of the developing tooth was devised, using antibody against antigen prepared from immature bovine enamel. Samples containing 5-500 ng of amelogenin protein in 50 microliters gave consistent results. Bovine enamelin cross-reacted with the antibody but was less reactive than amelogenin. Proteins in other tissues or fluids did not react to the antibody.