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Role of Cytokines Released During Pyroptosis in Non-Small Cell Lung Cancer

Yuanli Huang1,2, Guanghui Zhang1,2, Qing Zhu1

  • 1Department of Pathology, the First Affiliated Hospital of Bengbu Medical College, Bengbu City, Anhui Province, People's Republic of China.

Cancer Management and Research
|October 1, 2021
PubMed
Abstract

Insights

Gasdermin E (GSDME) expression in lung cancer tissues may predict patient survival. High GSDME levels indicate a better prognosis, while High Mobility Group Protein B1 (HMGB1) and CD8+ T cell roles require further study.

Area of Science:

  • Oncology
  • Cell Biology
  • Immunology

Background:

  • Pyroptosis is an inflammatory programmed cell death pathway involving Gasdermin proteins.
  • Cleaved Gasdermin E (GSDME) forms pores, releasing inflammatory factors like High Mobility Group Protein B1 (HMGB1).
  • Caspase-3 activation is crucial for GSDME cleavage and pyroptosis initiation.

Purpose of the Study:

  • To investigate the roles of GSDME, caspase-3, and HMGB1 in lung cancer.
  • To determine the clinical significance of these proteins in lung cancer tissues.
  • To analyze their association with patient prognosis and CD8+ T cell infiltration.

Main Methods:

  • Immunohistochemical staining was used to assess GSDME, caspase-3, and HMGB1 protein expression.
  • Expression levels were correlated with clinical stage, pathological grade, and survival.
  • CD8+ T cell accumulation was quantified and analyzed in relation to protein expression.

Main Results:

  • Significant differences in GSDME, caspase-3, and HMGB1 expression were observed between lung cancer and paracancerous tissues.
  • GSDME expression levels and lymph node invasion status were identified as prognostic indicators for lung cancer survival.
  • HMGB1 showed a correlation with lymph node metastasis but was not a reliable prognostic indicator.

Conclusions:

  • GSDME expression may serve as a significant prognostic biomarker for lung cancer survival.
  • The prognostic impact of HMGB1 expression and CD8+ T cell abundance in lung cancer warrants further investigation.
Keywords:
CD8+TGSDMEHMGB1caspase3cell pyroptosislung cancer

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