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Scanning Electron Microscope (SEM) Imaging to Determine Inflorescence Initiation and Development in Olive.
Amnon Haberman1, Einat Zelinger2, Alon Samach1
1The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Bio-Protocol
|October 1, 2021
Summary
This study details a scanning electron microscopy (SEM) protocol for imaging olive axillary bud meristems. The method aids in characterizing plant meristem identity and developmental stages for botanical research.
Area of Science:
- Plant Biology
- Microscopy Techniques
Background:
- Olive axillary bud meristems are crucial for plant development.
- Characterizing meristem identity and developmental stage requires advanced imaging.
Purpose of the Study:
- To present a detailed protocol for imaging olive axillary bud meristems using scanning electron microscopy (SEM).
- To enable precise characterization of meristem identity and developmental stage.
Main Methods:
- Specimen fixation with glutaraldehyde.
- Dehydration using an ethanol series.
- Drying via critical point drying (CPD).
- Dissection, conductive coating, and SEM imaging.
Main Results:
- Successful imaging of olive axillary bud meristem ultrastructure.
- Detailed visualization of meristem features relevant to identity and development.
Conclusions:
- The presented SEM protocol is effective for high-resolution imaging of olive axillary bud meristems.
- This method facilitates accurate assessment of meristem identity and developmental progression.
