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Updated: Oct 18, 2025
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Assaying the Kinase Activity of LRRK2 in vitro
Published on: January 18, 2012
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In vitro NLK Kinase Assay
Sungho Moon1, Jiyoung Kim1, Eek-Hoon Jho1
1Department of Life Science, University of Seoul, Seoul, Korea.
Bio-Protocol
|October 1, 2021
Abstract:
This protocol provides step by step instructions to perform an in vitro kinase assay for nemo-like kinase. In addition, this protocol also describes an efficient method using mild lysis buffer for expression and purification of Glutathione S-transferase (GST) fusion proteins.
