Quantification of Trypanosoma cruzi in Tissue and Trypanosoma cruzi Killing Assay
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In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase
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Hisako Kayama1,2,3, Shoko Kitada1,2,3, Kiyoshi Takeda1,2,3
1Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Osaka, Japan.
Bio-Protocol
|October 1, 2021
Abstract:
Infection with Trypanosoma cruzi causes Chagas disease. The methods provided here allow for the quantification of T. cruzi in the liver, heart, and blood of intraperitoneally-infected mice and analysis of the killing activity of the cells infected with T. cruzi in vitro.
