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Updated: Oct 18, 2025

Direct Tracheal Instillation of Solutes into Mouse Lung
Published on: August 29, 2010
Instillation of Particulate Suspensions to the Lungs
Etsushi Kuroda1,2, Yasuo Morimoto3, Ken J Ishii1,2
1Laboratory of Adjuvant Innovation, Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
Abstract:
Inhaled fine particulates are thought to cause chronic pulmonary inflammation through the deposition of particulates into the lungs. To investigate the effect of fine particulates on the lungs, instillation of suspension of particulates into the lungs is required. This protocol describes direct injection of suspensions of fine particulates into the airway. We also show examples of typical lung immune responses after particulate administration.
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