Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.5K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The MLLT3 YEATS domain binds histone marks (H3K9/18/27ac/cr) and ncRNA (7SK), linking transcription and RNA signaling to regulate hematopoiesis.

Cell reports·2026
Same author

Characterization and first application of a new <sup>236</sup>Np spike for (A)MS measurements of <sup>237</sup>Np.

Talanta·2026
Same author

Perioperative and Oncological Outcomes of Colorectal Cancer Surgery in Obese Patients: A Multicenter Retrospective Study.

Annals of gastroenterological surgery·2026
Same author

Timing and risk factors for peristomal granulation in pediatric tracheostomy patients.

International journal of pediatric otorhinolaryngology·2026
Same author

Clinical Impact of Nivolumab in Sinonasal Mucosal Melanoma: A 14-Year Single-Center Retrospective Study and Comprehensive Literature Review.

Cancers·2026
Same author

Impact of Aspirin on Recurrence Prevention After Curative Resection for Stage I-III Colorectal Cancer: A Multi-Institutional Retrospective Study.

Journal of surgical oncology·2026

Related Experiment Video

Updated: Oct 18, 2025

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome
06:39

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

Published on: October 3, 2018

9.9K

Myeloid Progenitor Transformation Assay.

Hiroshi Okuda1, Akihiko Yokoyama1

  • 1Tsuruoka Metabolomics Laboratory, National Cancer Center, Yamagata, Japan.

Bio-Protocol
|October 1, 2021
PubMed
Summary

Researchers detail an ex vivo murine myeloid transformation assay to study how oncogenes like mixed-lineage leukemia (MLL) gene fusions cause leukemia. This protocol helps characterize the transforming ability of these potent cancer-driving genes.

Keywords:
MLL fusionMixed lineage leukemiaMyeloidProgenitorTransformation

More Related Videos

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
07:21

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Published on: July 29, 2018

9.0K
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
14:37

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Published on: November 1, 2017

11.3K

Related Experiment Videos

Last Updated: Oct 18, 2025

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome
06:39

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

Published on: October 3, 2018

9.9K
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
07:21

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Published on: July 29, 2018

9.0K
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
14:37

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Published on: November 1, 2017

11.3K

Area of Science:

  • Hematology
  • Oncology
  • Molecular Biology

Background:

  • Leukemia development is often driven by oncogenes, including mixed-lineage leukemia (MLL) gene fusions generated through chromosomal translocations.
  • These MLL fusion proteins immortalize primary myeloid progenitors, contributing to leukemogenesis.
  • Characterizing the transforming potential of these oncogenes is crucial for understanding leukemia.

Purpose of the Study:

  • To provide a detailed protocol for an established ex vivo murine myeloid transformation assay.
  • To enable researchers to effectively study the transforming ability of leukemic oncogenes, particularly MLL fusions.

Main Methods:

  • The study details an ex vivo murine myeloid transformation assay.
  • This assay utilizes retroviral gene transduction to introduce oncogenes into primary myeloid progenitors.
  • The protocol has been refined over a decade for improved characterization of oncogene function.

Main Results:

  • The assay allows for the functional characterization of oncogenes in a controlled experimental setting.
  • Researchers can assess the immortalization capacity of specific gene products, such as MLL fusion proteins.
  • The detailed procedure facilitates reproducible experiments in leukemia research.

Conclusions:

  • The provided protocol offers a standardized method for evaluating the transforming activity of leukemic oncogenes.
  • This assay is essential for dissecting the molecular mechanisms underlying MLL-driven leukemias.
  • The established methodology aids in the ongoing research and therapeutic development for leukemia.