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Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells

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Published on: December 4, 2007

In vivo Leukemogenesis Model Using Retrovirus Transduction.

Hiroshi Okuda¹, Akihiko Yokoyama¹

¹Tsuruoka Metabolomics Laboratory, National Cancer Center, Yamagata, Japan.

|October 1, 2021

Summary

This study details a retroviral gene transduction protocol for a murine leukemia model. This model aids research into acute leukemia mechanisms driven by mixed-lineage leukemia (MLL) gene rearrangements.

Keywords:

Leukemia Leukemogenesis MLL fusion Mixed lineage leukemia Mouse model

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Published on: June 9, 2023

Area of Science:

Oncology
Genetics
Molecular Biology

Background:

Leukemia can arise from genetic alterations like chromosomal translocations.
Mixed-lineage leukemia (MLL) gene rearrangements create fusion genes that drive acute leukemia.
Murine models are crucial for studying leukemia development.

Purpose of the Study:

To provide a detailed protocol for a specific murine leukemia model.
To facilitate research on the mechanisms of acute leukemia onset.
To standardize the use of retroviral gene transduction in leukemia research.

Main Methods:

Retroviral gene transduction in mice.
Generation of MLL fusion genes.
Establishment of a murine leukemia model.

Main Results:

A reproducible protocol for a retroviral gene transduction-mediated murine leukemia model is presented.
The model allows for the study of oncogenic MLL fusion gene products.
This method has been widely used in leukemia research.

Conclusions:

The described protocol offers a valuable tool for investigating acute leukemia pathogenesis.
Standardized murine models are essential for advancing leukemia research.
Understanding MLL-driven leukemia mechanisms is critical for therapeutic development.