Measuring the Endocytic Recycling of Amyloid Precursor Protein (APP) in Neuro2a Cells

The established primary trigger of Alzheimer's disease's is β-amyloid (Aβ) (Mucke and Selkoe, 2012). Amyloid precursor protein (APP) endocytosis is required for Aβ generation at early endosomes (Rajendran and Annaert, 2012). APP retention at endosomes also depends on its recycling back to the plasma membrane ( Koo et al., 1996 ; Ubelmann et al., 2017 ). The following recycling assay has been optimized to assess APP recycling by live murine Neuro2a cells, a neuroblastoma cell line ( Ubelmann et al., 2017 ).