Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

14.4K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
14.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ActJK Signal Transduction System Contributes to Copper and Zinc Resistance in Sinorhizobium meliloti.

Environmental microbiology·2026
Same author

A systems-level insight into PHB-driven metabolic adaptation orchestrated by the PHB-binding transcriptional regulator AniA (PhaR).

mSystems·2025
Same author

A Survey of Annotated smORF-Encoded Polypeptides in Bacteria Interacting With Eukaryotic Hosts.

Proteomics·2025
Same author

Comparative Multi-Omics Survey Reveals Novel Specialized Metabolites and Biosynthetic Gene Clusters Under GacS Control in Pseudomonas donghuensis Strain SVBP6.

Molecular microbiology·2024
Same author

Small Regulatory RNAs of the Rsm Clan in Pseudomonas.

Molecular microbiology·2024
Same author

Identification and functional analysis of recent IS transposition events in rhizobia.

Mobile DNA·2024

Related Experiment Video

Updated: Oct 18, 2025

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
09:57

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

12.1K

Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry.

Antonio Lagares1, Claudio Valverde1

  • 1Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo, Universidad Nacional de Quilmes - CONICET, Bernal, Argentina.

Bio-Protocol
|October 1, 2021
PubMed
Summary

This study details a flow cytometry protocol to quantify intracellular polyhydroxybutyrate (PHB) in bacterial populations. The method refines previous techniques for accurate PHB measurement.

Keywords:
BacteriaFlow cytometryNile redPHBPolyhydroxybutyrate

More Related Videos

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

1.3K
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

20.7K

Related Experiment Videos

Last Updated: Oct 18, 2025

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
09:57

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

12.1K
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

1.3K
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

20.7K

Area of Science:

  • Microbiology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Polyhydroxybutyrate (PHB) is a key bacterial biopolymer with diverse applications.
  • Accurate quantification of intracellular PHB is crucial for strain development and metabolic engineering.
  • Existing methods for PHB quantification can be labor-intensive and variable.

Purpose of the Study:

  • To establish a standardized and detailed protocol for quantifying intracellular polyhydroxybutyrate (PHB) content in bacterial populations.
  • To leverage flow cytometry for high-throughput and precise PHB measurement.
  • To provide a consensus-based method integrating previous advancements.

Main Methods:

  • Development of a detailed flow cytometry protocol for PHB quantification.
  • Optimization of staining and gating strategies for bacterial cell analysis.
  • Validation of the protocol against established PHB measurement techniques.

Main Results:

  • A robust and reproducible flow cytometry protocol for intracellular PHB quantification was established.
  • The protocol allows for rapid assessment of PHB accumulation across a bacterial population.
  • The method demonstrates high sensitivity and specificity for PHB detection.

Conclusions:

  • Flow cytometry offers an efficient and accurate method for quantifying bacterial intracellular PHB.
  • This standardized protocol facilitates research in microbial biotechnology and biopolymer production.
  • The consensus-based approach ensures reliability and broad applicability.