Estimating combined health risks of nanomaterials and antibiotics from natural water: a proposed framework

Nanoparticles (NPs) are one of the major class of emerging contaminants identified in aquatic environment. There is a probability that they can co-exist with other chemical pollutants like antibiotics (ABs) as ABs-NPs complexes in natural water systems. If these complexes are taken up via inadvertent ingestion of contaminated water, it might show detrimental effects on human health. To address this challenging issue, this study developed a risk framework to assess the combined exposure of ABs and NPs in natural waters for the first time. The six-step framework was applied to a hypothetical exposure of NPs (copper oxide, CuO; zinc oxide, ZnO; iron oxide, Fe₃O₄; and titanium oxide, TiO₂) and ABs (ciprofloxacin, CIP; ofloxacin, OFX; norfloxacin, NOR; and levofloxacin, LEVO) to estimate human health risks for two different exposure scenarios. Risk estimation was also conducted for the released fragments of ABs, NPs and metal ions in the human digestive system. Mixture toxicity risk assessment was conducted for three different combinations: (i) ABs and metal ions, (ii) ABs and NPs, and (iii) NPs and metals ions. Although the expected risk values were observed to be less than 1 (both hazard quotients and hazard interactions less than 1) for all the conditions and assumptions made, still a thorough monitoring and analysis of the studied contaminants in water is required to protect humans from their adverse effects, if any. Maximum allowable concentrations (C_max) at which no risk can occur to humans was found to be (maximum values): ABs (233.8 µg/L, NOR); metal ions (1.02 × 10⁹ mg/L, Ti²⁺ ions), and NPs (6.68 × 10⁵ mg/L, TiO₂), respectively.