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Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
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Cyclization and Self-Assembly of Cyclic Peptides.

Alejandro Méndez-Ardoy1, Ignacio Insua1, Juan R Granja1

  • 1Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2021
PubMed
Summary

We developed on-resin methods for head-to-tail cyclic peptide synthesis. These cyclic peptides can self-assemble into nanotubes, showing potential for biomedicine and nanoelectronics.

Keywords:
Cyclic peptidesNanotubesSelf-assemblySolid-phase synthesisSupramolecular chemistry

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Area of Science:

  • Peptide chemistry
  • Supramolecular chemistry
  • Materials science

Background:

  • Cyclic peptides offer programmable folding and self-assembly into diverse structures.
  • Potential applications span biomedicine, nanoelectronics, and catalysis.
  • Controlled synthesis and characterization are key to harnessing their properties.

Purpose of the Study:

  • To describe on-resin procedures for head-to-tail cyclic peptide synthesis.
  • To present characterization methods for cyclic peptide assembly and dynamics.
  • To explore the formation of cyclic peptide nanotubes.

Main Methods:

  • On-resin peptide cyclization using orthogonal protecting groups.
  • Atomic Force Microscopy (AFM) for visualizing nanotube assembly.
  • Transmission Electron Microscopy (TEM) for high-resolution imaging.
  • Fluorescence microscopy for dynamic and structural analysis.

Main Results:

  • Successful implementation of on-resin head-to-tail cyclization.
  • Demonstration of cyclic peptide self-assembly into nanotubes.
  • Characterization of nanotube structures and dynamics using AFM, TEM, and fluorescence microscopy.

Conclusions:

  • On-resin synthesis provides an efficient route to cyclic peptides.
  • Cyclic peptides can self-assemble into nanotubes with potential applications.
  • Advanced microscopy techniques are essential for studying cyclic peptide assemblies.