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DeGlyPHER: An Ultrasensitive Method for the Analysis of Viral Spike N-Glycoforms
Sabyasachi Baboo1, Jolene K Diedrich1, Salvador Martínez-Bartolomé1
1Department of Molecular Medicine, The Scripps Research Institute, La Jolla, California 92037, United States.
Abstract:
Viruses can evade the host immune system by displaying numerous glycans on their surface "spike-proteins" that cover immune epitopes. We have developed an ultrasensitive "single-pot" method to assess glycan occupancy and the extent of glycan processing from high-mannose to complex forms at each N-glycosylation site. Though aimed at characterizing glycosylation of viral spike-proteins as potential vaccines, this method is applicable for the analysis of site-specific glycosylation of any glycoprotein.
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