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Updated: Oct 18, 2025

Test Samples for Optimizing STORM Super-Resolution Microscopy
Published on: September 6, 2013
Optimal Observables and Estimators for Practical Superresolution Imaging
Giacomo Sorelli1, Manuel Gessner1, Mattia Walschaers1
1Laboratoire Kastler Brossel, Sorbonne Université, ENS-Université PSL, CNRS, Collége de France, 4 Place Jussieu, F-75252 Paris, France.
Abstract:
Recent works identified resolution limits for the distance between incoherent point sources. However, it remains unclear how to choose suitable observables and estimators to reach these limits in practical situations. Here, we show how estimators saturating the Cramér-Rao bound for the distance between two thermal point sources can be constructed using an optimally designed observable in the presence of practical imperfections, such as misalignment, cross talk, and detector noise.
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