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Tagmentation-based single-cell genomics.

Andrew C Adey1

  • 1Department of Molecular & Medical Genetics, Knight Cancer Institute, Knight Cardiovascular Institute, Cancer Early Detection Advanced Research Center, Oregon Health and Science University, Portland, Oregon 97239, USA adey@ohsu.edu.

Genome Research
|October 2, 2021
PubMed
Summary

Tagmentation, a transposase-based DNA sequencing library preparation method, simplifies workflows and enables diverse single-cell genomics assays. This review explores tagmentation

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Tagmentation, a transposase-based method, simplifies DNA sequencing library preparation by simultaneously fragmenting DNA and adding adapter sequences.
  • This technique has become a preferred method due to its efficiency and simplicity, replacing multiple traditional steps.
  • Recent advancements have focused on single-cell applications, expanding the scope of molecular profiling.

Purpose of the Study:

  • To review tagmentation-based assays for single-cell genomics, focusing on the technology's components and properties.
  • To highlight how tagmentation enables innovative molecular tools for probing the single-cell regulatory landscape.
  • To provide a framework for understanding the diversity of tagmentation-based assay platforms.

Main Methods:

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  • Review of existing literature on transposase-based methods and tagmentation.
  • Analysis of tagmentation's central components and properties.
  • Categorization of tagmentation-based assays by their molecular profiling capabilities in single cells.

Main Results:

  • Tagmentation has revolutionized library preparation, offering a streamlined and efficient approach.
  • A wide array of tagmentation-based single-cell assays have been developed, targeting various regulatory elements.
  • The review emphasizes the enabling role of tagmentation's core features in assay innovation.

Conclusions:

  • Tagmentation is a versatile technology that has significantly advanced single-cell genomics.
  • Understanding tagmentation's properties is key to developing novel molecular tools for single-cell analysis.
  • This review aims to inspire future innovations in the field by outlining current assay diversity.