Related Experiment Video
Updated: Oct 18, 2025
07:38
Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin
Published on: May 6, 2018
8.6K
Summary
Producing polyclonal antibodies in small animals like mice is challenging due to low serum yield. Inducing ascites in mice significantly increases antibody yield, offering a viable alternative for antibody production.
Area of Science:
- Immunology
- Animal models in research
Background:
- Small laboratory animals (rats, mice, hamsters, guinea pigs) are commonly used for antibody production.
- Limited serum volume obtainable from these animals restricts the quantity of antibodies that can be produced.
Purpose of the Study:
- To address the challenge of low serum yield in small animals for polyclonal antibody production.
- To evaluate ascites fluid as a source of antibodies in mice.
Main Methods:
- Induction of ascites formation in mice.
- Collection of ascites fluid.
- Measurement of antibody titers in ascites fluid and comparison to serum titers.
Main Results:
- Ascites formation in mice provides a significantly larger volume of fluid compared to serum.
- Antibody titers in ascites fluid are comparable to those found in serum.
- This method allows for up to 10 mL of antibody-rich fluid per mouse.
Conclusions:
- Inducing ascites in mice is an effective method to overcome the limitation of low serum volume for polyclonal antibody production.
- Ascites fluid serves as a valuable and high-titer source for antibodies, making mice more suitable for large-scale production.
