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Updated: Oct 18, 2025

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Interferon-inducible protein, IFIX, has tumor-suppressive effects in oral squamous cell carcinoma

Shan Wang1,2, Fang Li3, Haixia Fan4

  • 1Department of Oral Pathology, Hospital of Stomatology, The First Affiliated Hospital, Harbin Medical University, Harbin, 150001, People's Republic of China. birchtree20032003@126.com.

Scientific Reports
|October 2, 2021
PubMed

Insights

Interferon-inducible HIN-200 family member IFIX suppresses oral cancer invasion by stabilizing the cytoskeleton. Higher IFIX expression correlates with better histopathological grade in oral squamous cell carcinoma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • The role of IFIX (interferon-inducible HIN-200 family) in oral cancer remains unclear.
  • IFIX is known as a tumor suppressor in breast cancer.

Purpose of the Study:

  • To investigate the function of IFIX in oral squamous cell carcinoma (OSCC).
  • To explore the relationship between IFIX expression and OSCC invasiveness and migration.

Main Methods:

  • Correlation analysis of IFIX expression with clinicopathological parameters in clinical OSCC samples.
  • In vitro studies using human tongue squamous cell carcinoma CAL-27 cells with IFIX overexpression.
  • Analysis of cytoskeletal proteins (cytokeratins) and paxillin expression.

Main Results:

  • Higher IFIX expression correlated significantly with histopathological grade in OSCC.
  • IFIX overexpression suppressed invasiveness and migration of CAL-27 cells.
  • IFIX-mediated suppression involved cytoskeleton stabilization via cytokeratins and paxillin downregulation.
  • IFIX did not affect cancer stem-like cell transformation in this model.

Conclusions:

  • IFIX exhibits tumor-suppressive functions in oral cancer.
  • IFIX stabilizes the cancer cell cytoskeleton, thereby inhibiting invasion and migration.
  • IFIX represents a potential therapeutic target for oral cancer.

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