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First Annotated Genome of a Mandibulate Moth, Neomicropteryx cornuta, Generated Using PacBio HiFi Sequencing
Xuankun Li1, Emily Ellis1, David Plotkin1
1McGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida, USA.
Genome Biology and Evolution
|October 2, 2021
Summary
We present a new genome assembly for Neomicropteryx cornuta, an archaic moth crucial for understanding early insect evolution. This high-quality genome aids research into the evolutionary transitions of Amphiesmenoptera (moths and caddisflies).
Area of Science:
- Genomics
- Evolutionary Biology
- Entomology
Background:
- Neomicropteryx cornuta belongs to Micropterigidae, an ancient moth lineage diverging over 300 million years ago.
- Understanding this lineage is key to deciphering the early evolution of Amphiesmenoptera, including Lepidoptera (butterflies and moths) and Trichoptera (caddisflies).
Purpose of the Study:
- To provide a new, high-quality, annotated genome assembly for Neomicropteryx cornuta.
- To establish a genomic resource for studying early Amphiesmenoptera evolution and ecological transitions.
Main Methods:
- PacBio HiFi sequencing for genome assembly.
- PacBio IsoSeq for isoform resolution and gene annotation.
- BUSCO analysis for assessing gene recovery.
Main Results:
- A highly contiguous genome assembly (contig N50 ~17 Mb) of 541,115,538 bp.
- High recovery of universal single-copy orthologs (98.1% BUSCO genes).
- Annotation of 15,643 genes, including resolved isoforms.
Conclusions:
- The genome assembly of Neomicropteryx cornuta is a valuable resource for evolutionary studies.
- This work facilitates research into the early diversification and evolutionary history of Amphiesmenoptera.
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