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Performance evaluation of automated cell counts compared with reference methods for body fluid analysis
Michela Seghezzi1, Giulia Previtali1, Valentina Moioli1
1Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy.
Diagnosis (Berlin, Germany)
|October 2, 2021
Summary
The UF-5000 analyzer demonstrates strong analytical performance for body fluid cell counts, offering a reliable alternative to optical microscopy for diagnosing various medical conditions.
Area of Science:
- Clinical Chemistry
- Hematology
- Medical Diagnostics
Background:
- Cellular analysis of body fluids (BFs) is crucial for diagnosing numerous medical conditions.
- Automated analyzers offer potential improvements in efficiency and accuracy for BF analysis.
Purpose of the Study:
- To evaluate the analytical performance of the UF-5000 body fluid mode (UF-BF) analyzer.
- To compare UF-BF performance against optical microscopy (OM) as the gold standard and the XN-1000 analyzer (XN-BF).
Main Methods:
- One hundred BF samples were analyzed using UF-BF, XN-BF, and OM.
- Agreement was assessed using Passing and Bablok regression and Bland-Altman analysis.
- Receiver operating characteristic (ROC) curves were used to evaluate diagnostic agreement.
Main Results:
- UF-BF showed good agreement with OM for cell counts, with slopes between 0.99-1.39 and biases ranging from 7.3%-52.9%.
- Comparison between UF-BF and XN-BF revealed slopes between 1.07-1.16 and biases from 5.8%-21.1%.
- ROC curve analysis indicated areas under the curve ranging from 0.9664 to 1.000.
Conclusions:
- The UF-BF analyzer exhibits very good performance for differential cell counts in ascitic, pleural, and synovial fluids.
- UF-BF is a valuable tool for screening and counting cells in these body fluid types.
