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Related Experiment Video

Updated: Oct 18, 2025

Counting Proteins in Single Cells with Addressable Droplet Microarrays
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High-throughput sorting of nanoliter droplets enabled by a sequentially addressable dielectrophoretic array.

Mun Hong Loo1, Yuta Nakagawa1, Soo Hyeon Kim2

  • 1Department of Chemistry, The University of Tokyo, Tokyo, Japan.

Electrophoresis
|October 2, 2021
PubMed
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High-throughput droplet sorting for large droplets is now possible. An upgraded sequentially addressable dielectrophoretic array (SADA) system achieves record speeds for 1 nanoliter (nL) droplet sorting, overcoming previous limitations.

Area of Science:

  • Microfluidics
  • Biotechnology
  • Biomedical Engineering

Background:

  • Droplet microfluidics is crucial for applications like single-cell analysis and metabolic engineering.
  • Droplet sorting isolates valuable contents but has been limited for larger droplet sizes.
  • Previous methods struggled with high-throughput sorting of 1 nanoliter (nL) droplets.

Purpose of the Study:

  • To develop a high-throughput method for sorting larger droplets (1 nL).
  • To overcome the throughput limitations of existing large droplet sorting techniques.

Main Methods:

  • Utilized an upgraded sequentially addressable dielectrophoretic array (SADA) system.
  • Increased the number of driving electrodes in the SADA.
  • Incorporated a slanted microchannel design for improved droplet manipulation.
Keywords:
DielectrophoresisDroplet microfluidicsDroplet sortingMicrofluidicsSingle-cell analysis

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Last Updated: Oct 18, 2025

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Main Results:

  • Achieved fluorescence-activated droplet sorting of 1 nL droplets at 1752 droplets/s.
  • This represents a record high throughput, doubling previous maximum rates for 1 nL droplets.
  • The upgraded SADA system effectively sorts larger droplets at unprecedented speeds.

Conclusions:

  • The enhanced SADA system with a slanted microchannel significantly advances large droplet sorting capabilities.
  • This breakthrough enables wider adoption of large droplet microfluidics in biomedical and industrial fields.
  • High-throughput sorting of 1 nL droplets is now feasible, unlocking new application potentials.