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Related Experiment Video

Updated: Oct 18, 2025

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Multivalent poultry vaccine development using Protein Glycan Coupling Technology.

Marta Mauri1, Thippeswamy H Sannasiddappa2, Prerna Vohra3,4

  • 1Department of Infection Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK.

Microbial Cell Factories
|October 3, 2021
PubMed
Summary
This summary is machine-generated.

This study developed a novel, low-cost live vaccine for poultry using bacterial vector technology to target Campylobacter jejuni, avian pathogenic E. coli (APEC), and Clostridium perfringens. While not reducing Campylobacter, the vaccine showed protection against APEC challenge.

Keywords:
APECCampylobacter jejuniGlycoconjugatesGlycoengineeringLive-attenuatedNetBOne HealthPGCTPoultryVaccine

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Area of Science:

  • Veterinary immunology
  • Microbial pathogenesis
  • Vaccine development

Background:

  • Global poultry production necessitates low-cost, multi-pathogen vaccines.
  • Glycoconjugate vaccines are effective but costly for livestock.
  • Protein Glycan Coupling Technology (PGCT) offers a cost-effective alternative.

Purpose of the Study:

  • To develop a novel glycan-based live vaccine for poultry.
  • To simultaneously target Campylobacter jejuni, avian pathogenic E. coli (APEC), and Clostridium perfringens.
  • To utilize bacterial cells as cost-effective vaccine factories.

Main Methods:

  • Engineered avian pathogenic E. coli (APEC) to express Campylobacter jejuni glycosylation genes.
  • Utilized the engineered APEC as a bacterial vector to deliver glycan antigens.
  • Constructed a multivalent vaccine expressing C. jejuni heptasaccharide and C. perfringens NetB toxoid.

Main Results:

  • Functional transfer of C. jejuni glycosylation locus into APEC resulted in mild attenuation.
  • The engineered APEC strain successfully delivered C. jejuni glycan antigens.
  • The vaccine conferred protection against homologous APEC challenge in chickens.
  • The vaccine did not significantly reduce Campylobacter colonization in the tested conditions.

Conclusions:

  • A candidate glycan-based multivalent live vaccine was generated for poultry.
  • The vaccine strategy shows potential for low-cost production of live-attenuated vaccine factories.
  • Further research may optimize efficacy against all targeted pathogens.