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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
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Cell-free DNA diagnostics in transplantation utilizing next generation sequencing.

Annette M Jackson1, Carly Amato-Menker2, Maria Bettinotti3

  • 1Duke University, Department of Surgery, DUMC Box 2645, Durham, NC 27710, USA.

Human Immunology
|October 3, 2021
PubMed
Summary

Next Generation Sequencing (NGS) of cell-free DNA (cfDNA) offers a minimally invasive biomarker for transplant diagnostics. Monitoring donor-derived cfDNA improves allograft health assessment, detecting injury, infection, and rejection.

Keywords:
BiomarkerCell free DNADNA sequencingRejectionTransplantation

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Area of Science:

  • Transplant diagnostics
  • Biomarker discovery
  • Molecular biology

Background:

  • Post-transplant monitoring of allograft health is critical.
  • Current methods may lack accuracy or invasiveness.
  • Cell-free DNA (cfDNA) presents a promising minimally invasive biomarker.

Purpose of the Study:

  • To evaluate the utility of Next Generation Sequencing (NGS) for interrogating cfDNA in transplant diagnostics.
  • To explore cfDNA as a biomarker for allograft health and injury.
  • To assess the potential of cfDNA monitoring for early detection of transplant complications.

Main Methods:

  • Isolation of cfDNA from various body fluids.
  • Interrogation of cfDNA using Next Generation Sequencing (NGS).
  • Bioinformatic analysis to determine cfDNA origins and characteristics.

Main Results:

  • cfDNA serves as a powerful, minimally invasive biomarker for disease and tissue injury.
  • Donor-derived cfDNA monitoring can identify active allograft injury, infection, and rejection.
  • cfDNA characteristics provide insights into its generation and release mechanisms.

Conclusions:

  • NGS-based cfDNA interrogation is a crucial advancement in post-transplant monitoring.
  • cfDNA offers a minimally invasive, quantitative, and reproducible measure of allograft injury.
  • Further evaluation of cfDNA detection methods holds promise for widespread clinical application across organ types.