Chronic ozone exposure preferentially modifies root rather than foliar metabolism of date palm (Phoenix dactylifera)...

In their natural environment, date palms are exposed to chronic atmospheric ozone (O₃) concentrations from local and remote sources. In order to elucidate the consequences of this exposure, date palm saplings were treated with ambient, 1.5 and 2.0 times ambient O₃ for three months in a free-air controlled exposure facility. Chronic O₃ exposure reduced carbohydrate contents in leaves and roots, but this effect was much stronger in roots. Still, sucrose contents of both organs were maintained at elevated O₃, though at different steady states. Reduced availability of carbohydrate for the Tricarboxylic acid cycle (TCA cycle) may be responsible for the observed reduced foliar contents of several amino acids, whereas malic acid accumulation in the roots indicates a reduced use of TCA cycle intermediates. Carbohydrate deficiency in roots, but not in leaves caused oxidative stress upon chronic O₃ exposure, as indicated by enhanced malonedialdehyde, H₂O₂ and oxidized glutathione contents despite elevated glutathione reductase activity. Reduced levels of phenolics and flavonoids in the roots resulted from decreased production and, therefore, do not indicate oxidative stress compensation by secondary compounds. These results show that roots of date palms are highly susceptible to chronic O₃ exposure as a consequence of carbohydrate deficiency.