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Monoterpenoid biosynthesis by engineered microbes.

Yurou Liu1,2, Xiaoqiang Ma1, Hong Liang1,2

  • 1Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117585, Singapore.

Journal of Industrial Microbiology & Biotechnology
|October 3, 2021
PubMed
Summary
This summary is machine-generated.

Microbial biosynthesis offers a promising alternative for producing valuable monoterpenoids. Metabolic engineering strategies are advancing microbial production, overcoming challenges like volatility and cytotoxicity for commercial viability.

Keywords:
Escherichia coliSaccharomyces cerevisiaeBiotransformationMetabolic engineeringMicrobial productionMonoterpenoids

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Area of Science:

  • Biotechnology
  • Natural Products Chemistry
  • Metabolic Engineering

Background:

  • Monoterpenoids are C10 isoprenoids widely used in food, cosmetics, and therapeutics.
  • Traditional extraction methods from plants are costly, laborious, and yield low purity.
  • Microbial biosynthesis presents a sustainable and scalable alternative for monoterpenoid production.

Purpose of the Study:

  • To review recent advances in microbial production of monoterpenoids.
  • To highlight key metabolic engineering strategies for enhancing monoterpenoid yields.
  • To provide insights into future prospects for industrial-scale microbial production.

Main Methods:

  • Review of literature on microbial biosynthesis of monoterpenoids over the past three years.
  • Analysis of metabolic engineering approaches applied to microbial hosts.
  • Discussion of challenges including monoterpenoid volatility, enzymatic limitations, and product toxicity.

Main Results:

  • Significant progress has been made in engineering microbial hosts for monoterpenoid production.
  • Metabolic engineering strategies have addressed issues of low enzymatic activity and product cytotoxicity.
  • Overcoming monoterpenoid volatility remains a key challenge for efficient recovery.

Conclusions:

  • Microbial production of monoterpenoids is a rapidly advancing field with commercial potential.
  • Continued metabolic engineering efforts are crucial for improving productivity and overcoming current limitations.
  • Future research should focus on integrated strategies for enhanced yield and economic feasibility.