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Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture
Francisco Carriquiriborde1, Pablo Martin Aispuro1, Nicolás Ambrosis1
1Laboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, Argentina.
Frontiers in Immunology
|October 4, 2021
Summary
Outer membrane vesicles (OMVs) from biofilm-grown Bordetella pertussis offer enhanced protection against pertussis, including against pertactin-deficient strains. This biofilm-derived OMV vaccine elicits a robust immune response, addressing limitations of current vaccines.
Area of Science:
- Immunology
- Microbiology
- Vaccinology
Background:
- Pertussis, caused by Bordetella pertussis, is a resurgent disease necessitating improved vaccines.
- Current acellular pertussis vaccines have limitations, and new strains are emerging, including pertactin-deficient (PRN(-)) variants.
- Outer membrane vesicles (OMVs) from B. pertussis show promise for vaccine development, inducing mixed Th1/Th2/Th17 and tissue-resident memory responses.
Purpose of the Study:
- To evaluate the immunogenicity and protective efficacy of OMVs derived from B. pertussis grown in a biofilm.
- To compare the protective capacity of biofilm-derived OMVs (OMVbiof) against planktonic-derived OMVs (OMVplank) against both PRN(+) and PRN(-) B. pertussis strains.
- To assess the potential of OMVbiof as a third-generation pertussis vaccine.
Main Methods:
- B. pertussis clinical isolates with biofilm-forming capacity were cultured in biofilm and planktonic conditions.
- Biofilm formation was confirmed using scanning electron microscopy and proteomics.
- OMVs were extracted from both culture types, formulated into vaccines, and tested in a mouse protection model against PRN(+) and PRN(-) B. pertussis isolates.
Main Results:
- OMVbiof vaccines were more immunogenic than OMVplank vaccines, inducing higher specific antibody titers and avidity.
- OMVbiof vaccines provided significantly higher protection against both PRN(+) and PRN(-) B. pertussis strains, even at suboptimal doses.
- Biofilm culture conditions led to overexpression of biofilm-associated factors like BipA and fimbriae in B. pertussis.
Conclusions:
- B. pertussis biofilm-derived OMVs are a promising candidate for next-generation pertussis vaccines.
- OMVbiof vaccines induce a robust immune response and offer broad protection, including against circulating PRN(-) strains.
- Utilizing biofilm-derived OMVs represents a strategic approach to overcome the limitations of current pertussis vaccines.
