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Morphological analysis and immunohistochemical expression in restorations with self-adhesive resin: A randomized
Natália-Gomes de Oliveira1, Marina-Torreão da Silveira2, Leorik-Pereira da Silva3
1MSc, PhD student, Dental School, Universidade de Pernambuco/FOP-UPE; Camaragibe, PE, Brazil.
Journal of Clinical and Experimental Dentistry
|October 4, 2021
Summary
Both self-adhesive and conventional resin composites caused minor changes in the dentin-pulp complex. These findings suggest the dental pulp can repair itself after restorative procedures.
Area of Science:
- Dentistry
- Biomaterials Science
- Oral Biology
Background:
- Dental restorations aim to preserve tooth structure and function.
- Assessing the biological response of the dentin-pulp complex to new restorative materials is crucial.
- Understanding the inflammatory and remodeling processes initiated by dental composites is essential for long-term clinical success.
Purpose of the Study:
- To compare the effects of a self-adhesive composite (Vertise Flow) versus a conventional resin composite with a self-etching adhesive (Filtek Z250/Clearfil SE Bond) on the dentin-pulp complex.
- To evaluate the morphology and immunohistochemical expression of matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-3 (TIMP-3), collagen type I (COL-I), tumor necrosis factor-alpha (TNF-alfa), and cyclooxygenase-2 (COX-2).
Main Methods:
- A randomized, controlled, double-blind, split-mouth clinical trial involving 27 volunteers.
- Deep class I restorations were placed using either Vertise Flow or a combination of Filtek Z250/Clearfil SE Bond in third molars indicated for extraction.
- Morphological analysis and immunohistochemical expression of specific biomarkers were assessed 15 days post-restoration.
Main Results:
- Morphological analysis revealed slight disorganization of the odontoblastic layer and mild inflammatory infiltrate in both restorative groups (p<0.05).
- Immunohistochemical analysis showed increased positive pulp labeling for TNF-alpha, MMP-9, TIMP-3, and COX-2 in both groups.
- No significant differences were noted between the self-adhesive and conventional composite groups in the evaluated parameters.
Conclusions:
- Both self-adhesive resin composite and conventional resin composite with self-etching adhesive induced minimal alterations in the dentin-pulp complex.
- The observed changes suggest a capacity for repair and reversibility of the inflammatory process following adhesive restorative procedures.
- These findings support the biocompatibility of both restorative approaches in the short term.
