Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

8.7K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.7K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

142
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
142
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.1K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.1K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.3K
3.3K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

219
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
219
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

256
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
256

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of an AsnC/Lrp-family transcriptional regulator in <i>Herbaspirillum rubrisubalbicans</i> M1: linking plant interaction, nitrogen response and PHB metabolism.

Frontiers in microbiology·2026
Same author

Ancestral Protein Reconstruction Uncovers a Thermotolerant Rieske Oxygenase with Enhanced <i>O</i>-Demethylation Activity toward 3-<i>O</i>-Methylgallate.

ACS synthetic biology·2026
Same author

Improving a Tn7-based luciferase reporter system for promoter activity studies.

Microbiology (Reading, England)·2026
Same author

A Hanks-type bacterial kinase, PknS, directly phosphorylates the alternative sigma factor EcfK to promote resistance to protist predation.

The FEBS journal·2026
Same author

NifFinder: improved Nif protein prediction using SWeeP vectors and neural networks.

Bioinformatics advances·2025
Same author

Impact of long COVID phenotypes on quality of life following symptomatic omicron infection in Brazil: a machine learning analysis.

BMC infectious diseases·2025

Related Experiment Video

Updated: Oct 18, 2025

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.5K

Control of Gene Expression With Quercetin-Responsive Modular Circuits.

Fernanda Miyuki Kashiwagi1, Brenno Wendler Miranda2, Fabio de Oliveira Pedrosa3

  • 1Postgraduate Program in Science (Biochemistry), Department of Biochemistry and Molecular Biology, Nitrogen Fixation Laboratory, Federal University of Paraná (UFPR), Curitiba, Brazil.

Frontiers in Bioengineering and Biotechnology
|October 4, 2021
PubMed
Summary

Researchers engineered controllable genetic circuits in E. coli using the QdoR system activated by the plant metabolite quercetin. This allows precise tuning of gene expression levels and noise for biotechnological applications.

Keywords:
E. coliQdoRflavonoidgenetic circuitquercetin

More Related Videos

Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

1.7K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.0K

Related Experiment Videos

Last Updated: Oct 18, 2025

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.5K
Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

1.7K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.0K

Area of Science:

  • Synthetic biology
  • Metabolic engineering
  • Bacterial genetics

Background:

  • Controllable gene expression is vital for genetic circuits in biotechnology.
  • Transcriptional regulators activated by specific, orthogonal signals are needed.
  • Plant metabolites like flavonoids are potential signals for bacterial gene control.

Purpose of the Study:

  • To decompose and reassemble the QdoR regulatory system from B. subtilis for use in E. coli.
  • To create quercetin-inducible genetic circuits with tunable expression and noise.
  • To characterize the performance of QdoR-regulated genetic parts in E. coli.

Main Methods:

  • Decomposition and reassembly of the QdoR regulatory system.
  • Engineering of genetic circuits with varying QdoR expression levels.
  • Characterization of promoter activity and gene reporter expression in E. coli.
  • Analysis of gene expression noise in response to quercetin concentration.

Main Results:

  • Identified a functional QdoR-regulated promoter in E. coli.
  • Constructed quercetin-inducible circuits with distinct gene expression levels.
  • Demonstrated that QdoR expression levels inversely affect reporter gene expression.
  • Showed that quercetin concentration influences gene expression noise.

Conclusions:

  • The QdoR system can be repurposed to create tunable genetic circuits in E. coli.
  • Quercetin serves as an effective and natural inducer for these engineered circuits.
  • These circuits offer potential for applications requiring adjustable gene expression and noise levels.