Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

pre-mRNA Processing02:01

pre-mRNA Processing

54.3K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
54.3K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

11.0K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
11.0K
RNA Splicing01:32

RNA Splicing

57.8K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
57.8K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.0K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.0K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

12.4K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
12.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multicenter external validation of normal tissue complication probability models for radiation-induced primary hypothyroidism in head and neck cancer survivors with long-term endocrine outcomes.

International journal of radiation oncology, biology, physics·2026
Same author

Integrative single-cell and spatial transcriptomic analysis reveals that ATG13 enriched in cancer-associated fibroblasts is associated with the suppression of ferroptosis in colorectal cancer.

Discover oncology·2026
Same author

InnerEye-HS: a disease-agnostic clinical tool for hippocampal segmentation.

Brain communications·2026
Same author

Al─N Co-Doped LLZO Solid Electrolytes via One-Step Sintering: Toward High Ionic Conductivity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Multi-omics biomarkers in endometrial receptivity: from mechanisms to clinical translation.

Journal of translational medicine·2026
Same author

Quality and consistency of genetic newborn screening reports in China: insights from a multi-laboratory analysis using simulated samples.

BMC pediatrics·2026

Related Experiment Video

Updated: Oct 18, 2025

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

12.9K

U2.3 Precursor Small Nuclear RNA in vitro Processing Assay.

Chan Lin1, Yujie Feng1, Xueyan Peng1

  • 1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China.

Bio-Protocol
|October 4, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel in vitro method to study precursor small nuclear RNA (pre-snRNA) 3' end processing. This breakthrough clarifies the maturation of essential pre-snRNA molecules, crucial for eukaryotic cell function.

Keywords:
ArabidopsisIn vitroPre-snRNAProcessingsnRNA

More Related Videos

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.9K
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

7.5K

Related Experiment Videos

Last Updated: Oct 18, 2025

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

12.9K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.9K
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

7.5K

Area of Science:

  • Molecular Biology
  • RNA Processing
  • Eukaryotic Gene Expression

Background:

  • Small nuclear RNAs (snRNAs) are essential for pre-mRNA splicing in eukaryotes.
  • Transcription mechanisms of snRNA are well-studied using cell-free systems.
  • The 3' end maturation of precursor snRNA (pre-snRNA) processing remains poorly understood due to assay limitations.

Purpose of the Study:

  • To establish a robust in vitro assay for studying pre-snRNA 3' end cleavage.
  • To investigate the molecular mechanisms governing pre-snRNA maturation.

Main Methods:

  • Development of an in vitro processing assay using synthetic labeled RNA substrates.
  • Utilizing the assay to analyze the 3' cleavage of pre-snRNA.

Main Results:

  • Successfully established a functional in vitro system for pre-snRNA 3' end processing.
  • The assay enables detailed biochemical analysis of pre-snRNA maturation.

Conclusions:

  • The developed in vitro method overcomes previous limitations in studying pre-snRNA processing.
  • This assay provides a valuable tool for future research into snRNA biogenesis and function.