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Cytoduction and Plasmiduction in Yeast.

Jane E Dorweiler1, Anita L Manogaran1

  • 1Department of Biological Sciences, Marquette University, Milwaukee, WI, USA.

Bio-Protocol
|October 4, 2021
PubMed
Summary

Cytoduction and plasmiduction offer streamlined methods for transferring yeast prions between strains. These techniques simplify prion studies by enabling efficient transfer and verification of these non-Mendelian cytoplasmic elements.

Keywords:
CytoductionHeterokaryonKaryogamyPlasmiductionPrionYeastkar1

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Area of Science:

  • Yeast genetics
  • Molecular biology
  • Prion biology

Background:

  • Prions are protein aggregates exhibiting non-Mendelian inheritance.
  • Traditional methods for prion transfer are complex or yield non-isogenic strains.

Purpose of the Study:

  • To present cytoduction and plasmiduction as efficient techniques for yeast prion transfer.
  • To enable streamlined research in yeast prion biology.

Main Methods:

  • Cytoduction utilizes a kar1 mutation to prevent nuclear fusion during yeast mating.
  • Plasmiduction allows transfer of plasmids encoding fluorescently tagged prion proteins.
  • Modified protocols accommodate slow-growing or respiratory-deficient strains.

Main Results:

  • Cytoduction successfully transfers prions to recipient yeast strains.
  • Plasmiduction enables visual verification of prion aggregates via fluorescent tagging.
  • The techniques are adaptable to various yeast strain types.

Conclusions:

  • Cytoduction and plasmiduction significantly advance yeast prion research.
  • These methods provide a robust and adaptable platform for studying prion inheritance and propagation.