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Extended leukocyte parameters for hematology analyzers.

D Peebles, S Kirchanski, M Brown

Annals of the New York Academy of Sciences

|January 1, 1986

Summary

New hematology analyzers identify normal leukocyte samples, reducing manual analysis. This allows labs to focus resources on abnormal specimens, improving efficiency.

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Area of Science:

Hematology
Clinical Pathology
Laboratory Medicine

Background:

Extended leukocyte parameters are now available on laser-based hematology analyzers.
Current methods often require manual analysis of all samples, consuming valuable laboratory resources.

Purpose of the Study:

To evaluate a new automated method for identifying and excluding normal leukocyte samples from manual analysis.
To assess the efficiency and accuracy of a novel reagent and optical system for leukocyte differential counting.

Main Methods:

Utilized a new reagent to lyse erythrocytes, preserving leukocyte optical properties.
Employed a specialized optical bench to measure narrow-angle forward light scatter (cell size) and wide-angle light scatter (cell granularity).
Developed a microprocessor algorithm to analyze scatter data, generate histograms, and differentiate leukocyte populations (lymphocytes, monocytes, granulocytes).

Main Results:

The system successfully delineated lymphocytes, monocytes, and granulocytes on histograms.
Microprocessor analysis accurately set thresholds and produced three-part leukocyte counts.
Clinical studies showed very good correlation between automated counts and manual leukocyte differentials.
The method demonstrated significant potential for reducing the number of manual differentials required for normal samples.

Conclusions:

Extended leukocyte parameters on laser-based analyzers can effectively identify normal samples for exclusion from manual review.
This technology enhances laboratory efficiency by enabling a focus on abnormal specimens.
The new reagent and optical system provide accurate and reliable leukocyte differential counts.

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