Related Experiment Video
Updated: Oct 18, 2025
Isolation of Human Neutrophils from Whole Blood and Buffy Coats
Published on: September 17, 2021
Isolation of polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood
Chang Cui1,2, Kelly Q Schoenfelt2, Kristen M Becker2
1Commitee on Cancer Biology, The University of Chicago, Chicago, IL 60637, USA.
Monocytes and neutrophils are widely distributed throughout the body and play essential roles in health and disease. Here, we present a detailed protocol to isolate polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood. We have optimized several aspects of the procedure, including the density gradient, timing of each cell processing step, and the buffer/media conditions to preserve cell viability for subsequent functional assays. This protocol is reproducible and can be scaled as required for downstream applications. For complete details on the use and execution of this protocol, please refer to Cui et al. (2021).
Monocytes and neutrophils are widely distributed throughout the body and play essential roles in health and disease. Here, we present a detailed protocol to isolate polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood. We have optimized several aspects of the procedure, including the density gradient, timing of each cell processing step, and the buffer/media conditions to preserve cell viability for subsequent functional assays. This protocol is reproducible and can be scaled as required for downstream applications. For complete details on the use and execution of this protocol, please refer to Cui et al. (2021).
